NIZO food research has an established microcolon model that allows the high-throughput screening of novel ingredients and probiotics for their efficacy in the gastrointestinal tract. The microcolon model is inoculated with fecal slurries derived from either infants or adults and, when desired, can be spiked with specific pathogens of interest to our customers. A fermentation medium and conditions mimicking the human gastrointestinal tract are employed. We are able to routinely perform DNA isolations, qPCR analyses to monitor specific species, analyses of organic acids, as well as amplicon library preparation and establishment of the full microbiota composition. These technologies allow our customers to rapidly benchmark their new ingredient, or select the most promising ingredients from large panels to rapidly move into a preclinical phase with a limited number of ingredients.