Industry perspectives

In this series of articles, Will Chu, Science Editor for NutraIngredients, discusses some of the key issues and challenges facing the nutraceutical and food ingredient industry today.

By 2050, the global population may reach 10 billion people. Feeding this booming humanity will require increasing food production by more than 60% – placing intense pressure on resources including arable land and fresh water. Food and ingredient companies are thus turning an interested eye towards microbial biomass as a potential sustainable source of protein. Yet, as Anita Hartog, Senior Scientist at NIZO, explains, sustainability is only one side of the story for edible biomass. It’s time to look at the potential nutritional and health benefits, as well.

Will Chu: What is microbial biomass, and why is it interesting for food and ingredient manufacturers?

Anita Hartog: The term ‘microbe’ is used here in the broad connotation of bacteria, fungi, yeast and algae. Microbial biomass is the result of the fermentation, by these microbes, of organic substrates such as by-products from the sugar industry and other food processing residues, or hydrogen substrates including methanol and methane. Biomass suitable for human or animal consumption has often been referred to as single cell protein (SCP).

Using these types of edible microorganisms isn’t a completely new concept; during World War I, for example, Germany produced edible yeast for food on a large scale. But the idea of using microbial biomass as a food source really started gaining steam in the 1960s, eventually resulting, for example, in the 1980s launch of mycoprotein (Quorn), derived from the Fusarium venenatum fungus. The primary incentive for using microbial biomass to produce food has been its sustainability: it doesn’t require as much arable land or water as raising animals and plants. And it can transform side streams or even discarded substrates into protein, reducing wastage. But microbial biomass can offer consumers and producers much more.

Figure 1A
Figure 1 The environmental impact of food/ protein production is high: the upper table shows the land needed to produce 1 kg protein, while the lower graph indicates the CO2 emissions from the production of 1 kg protein. Data are adapted from Ciani, 2021.[1]


[1] Ciani M, Lippolis A, Fava F, Rodolfi L, Niccolai A, Tredici MR: Microbes: Food for the Future. Foods 2021, 10.
 

WC: Is microbial biomass protein as high in protein as meat and dairy?

AH: The protein content of algae, such as spirulina, can be as high as 46-65% of its dry weight. Fungi can run to 30-50% protein: mycoprotein, for example, is 45% protein by dry weight. Bacteria can be 50-80% protein. And yeast can have a protein content of around 50% protein by dry weight.

These amounts compare well to meat and dairy, and are even higher than many plant-based alternatives.

However, protein quantity isn’t the whole story: you have to consider its quality and bioavailability as well. Can the protein be used by the human body?

WC: How do you determine the quality of microbial biomass protein?

AH:  To do this, you need to look at the amino acid composition and digestibility of the proteins. Amino acids are important for growth, tissue repair, and various other functions. As a benchmark, animal protein contains all nine essential amino acids.

The amino acid profiles for microbial proteins in fact compare very well with meat and fish, and with the standard requirements for human consumption as defined by the FAO/WHO. Plant proteins, on the other hand, generally lack or are low in some of these essential amino acids.

But these amino acids also need to be ‘available’ for the body to use: this is where digestibility comes in. Digestion starts in the mouth, with mastication and saliva, and continues in the stomach (with the gastric juices) and the intestines (with bile, pancreatic juice and intestinal juice). Different proteins and protein fractions will be degraded at different rates during the various digestive phases, as the amino acid chains are broken down into individual amino acids. The latter can be absorbed in the small intestine, and released into the blood stream, which delivers them throughout the body.

The digestibility of microbial biomass proteins varies. Fungi, for example, have a digestibility similar to eggs and milk, whereas algae is less digestible. By breaking down the algae’s cell walls, its digestibility can be increased.

Figure 2
In vitro digestions models can simulate the salivary, gastric and intestinal phase of adult or infant digestion. Protein degradation can be analysed using the o-phthaldialdehyde (OPA, NH2) method, which is based on the measurement of amino groups generated from during the digestion. This figure depicts the digestibility of 3 different protein fractions.

WC: What other nutritional value or health benefits can microbial biomasses offer?

AH: Edible microbial biomasses can be excellent vegan sources of a range of vitamins, fats, fibres, etc. For example, K2 vitamins, which are generally not found in plants, can be produced by certain bacteria. K2 plays a role in blood clotting, bone health and heart health.

Algae, on the other hand, can be a good source of the omega 3 fatty acids EPA and DHA. These are very important for the structure of the body’s cell walls, and can affect the heart, lungs, blood vessels and immune system. Omega 3 fatty acids like EPA and DHA are most often provided by fish, so it is very interesting to have a vegan source.

The fibres from the microbial biomass can also provide additional health benefits. In this context, yeast and algae can be sources of beta glucans, which have been shown to impact immune functions.

WC: How do you choose a cellular organism or substrate for producing biomass for use in foods?

AH: The current rationale for exploring edible microbial biomass is as a sustainable protein source. But within that context, you need to ensure you have a viable protein product.

How easy it is to cultivate the substrate, but also to isolate the protein? With algae, for example, you have to break down the cell wall, but for fungi and bacteria you don’t: what will be the impact of this additional step?

The functional characteristics of the protein are also important. Is it soluble, does it emulsify, will it foam? What types of products can you use it in? Is it digestible? What processing steps will you need to take to turn it into a viable ingredient?

Finally, you also have to consider your own branding. If you position yourself and your products as the ‘sustainable’ choice, you may focus on proteins that meet that need, while delivering the functional characteristics to create an appealing product. But if your brand is more about ‘high nutrition’, then you will want to find a microbial biomass with the quality and digestibility to match the expectations of your customers.

WC: What is in the future for microbial proteins as a food or ingredient?

AH: Microbial biomass remains an undiscovered country: there is still a lot to explore, and there may be many additional nutritional and health benefits to uncover.

In addition, there could be potential for including both microbial proteins and plant-derived proteins in a food product, to combine their strengths.

But clearly, microbial proteins offer a lot of potential, as we seek to create greater nutritional and health value, using fewer natural resources.